MISSION® siRNA Druggable Genome Libraries

The MISSION® siRNA Performance Guarantee*
Sigma guarantees that 2 out of 3 siRNA duplexes per target gene will achieve knockdown efficiencies of greater than 75%.

MISSION® siRNA Library Format:

• 21mer siRNA duplexes with TT overhangs
• 3 individual siRNA duplexes per target gene designed using Rosetta Inpharmatics' design algorithm
• All siRNA duplexes spotted in 96 well microplates with 80 duplexes per plate. First and last columns of each plate are empty.
• Control siRNA sequences included on all plates

Overview
Sigma and Rosetta Inpharmatics, a recognized leader in Bioinformatics, have partnered to take your RNAi research to the next level.

Current studies suggest that the rules used to design gene-specific siRNAs have a direct effect on how well your siRNA will perform in a given RNAi experiment. A good siRNA design reduces off-target effects and leads to down regulation of all types of genes, not simply the more abundant high expressors. Using an siRNA designed with a best-in-class algorithm saves time and money, allowing you to focus on downstream applications, not up-front siRNA design work.

The Rosetta-powered siRNA design algorithm used by Sigma to design MISSION® siRNA druggable genome libraries has been optimized with over 3 years of continuous development for enhanced performance in RNAi applications. New and critical rules incorporated into the latest design algorithm lead to increased target specificity and knockdown for low abundance messages.

Figure 1: Silencing Efficacy of 20 MISSION® siRNAs designed using the Rosetta algorithm. Target mRNA levels were measured by Quantigene from samples harvested 24 hr after transfection into HeLa cells.

MISSION® siRNA Library Format:

• 21mer siRNA duplexes with TT overhangs
• 3 individual siRNA duplexes per target gene designed using Rosetta Inpharmatics' design algorithm
• All siRNA duplexes spotted in 96 well microplates with 80 duplexes per plate. First and last columns of each plate are empty.
• Control siRNA sequences included on all plates

To order individual siRNAs, visit the Genosys/Proligo homepage

MISSION® siRNA Human Druggable Genome

MISSION® siRNA Rat Druggable Genome

MISSION siRNA Mouse Kinase Library